Pedersen N T, Marqversen J, Skjoldborg H, Jensen E
Scand J Gastroenterol. 1979;14(5):529-34. doi: 10.3109/00365527909181385.
A double-tracer technique to estimate lipid digestion was investigated. 3H-labelled oleic acid and 14C-labelled triolein were ingested in a test meal. The serum radioactivity of 3H after ingestion of labelled oleic acid depends on absorption and metabolism of free fatty acids, while serum radioactivity of 14C from triolein, in addition to the former, depends on triglycerol digestion. This study shows that the ratio between 3H and 14C 2h after the test meal gives a good qualitative and quantitative estimation of lipid digestion: the 3H/14C ratio in patients with maldigestion is significantly higher than for normals (P less than 0.01), the predictive value of the 3H/14C ratio in the diagnosis of maldigestion is high, that of a positive result being 1.0 and that of a negative 0.93, and quantitatively the 3H/14C ratio shows a highly significant correlation with faecal fat (P less than 0.001). The test is very easy to perform, lasts for only 2 h, and is without discomfort to the patient or nursing and laboratory staff. It gives information like or superior to that of faecal fat measurement.
研究了一种用于估计脂质消化的双示踪技术。在一次试验餐中摄入了3H标记的油酸和14C标记的三油酸甘油酯。摄入标记油酸后血清中3H的放射性取决于游离脂肪酸的吸收和代谢，而来自三油酸甘油酯的14C的血清放射性，除了前者外，还取决于甘油三酯的消化。本研究表明，试验餐后2小时3H与14C的比值能对脂质消化进行良好的定性和定量估计：消化不良患者的3H/14C比值显著高于正常人（P<0.01），3H/14C比值在诊断消化不良中的预测价值很高，阳性结果的预测值为1.0，阴性结果的预测值为0.93，并且定量地3H/14C比值与粪便脂肪显示出高度显著的相关性（P<0.001）。该试验操作非常简便，仅持续2小时，且对患者、护理人员和实验室工作人员均无不适。它提供的信息与粪便脂肪测量相当或更优。
