Reiter H, Strauss B, Robbins M, Marone R
J Bacteriol. 1967 Mar;93(3):1056-62. doi: 10.1128/jb.93.3.1056-1062.1967.
A nuclease present in extracts of Bacillus subtilis inserts breaks in deoxyribonucleic acid (DNA) treated with the monofunctional alkylating agent, methyl methanesulfonate (MMS), but the nature of the sites within the alkylated macromolecule at which these breaks occur is not known. DNA extracted from B. subtilis cells that have recovered from MMS damage has lost its susceptibility to enzyme action. The recovery process is accompanied by some DNA breakdown and by the incorporation of thymidine. Some recovery from ultraviolet irradiation (UV) and MMS occurred in organisms starved for thymine or adenine, but UV recovery was stimulated by their addition. It is possible that MMS recovery proceeds by a process of excision and repair similar to, but not identical with, UV repair.
枯草芽孢杆菌提取物中的一种核酸酶能使经单功能烷基化剂甲磺酸甲酯（MMS）处理的脱氧核糖核酸（DNA）产生断裂，但尚不清楚这些断裂发生在烷基化大分子内的哪些位点。从遭受MMS损伤后恢复的枯草芽孢杆菌细胞中提取的DNA，已失去了对酶作用的敏感性。恢复过程伴随着一些DNA降解以及胸腺嘧啶的掺入。在缺乏胸腺嘧啶或腺嘌呤的生物体中，紫外线照射（UV）和MMS造成的损伤会有一定程度的恢复，但添加这些物质会促进UV损伤的恢复。MMS损伤的恢复过程有可能是通过一种类似于UV修复但又不完全相同的切除和修复过程来进行的。
