Verma D S, Fisher R, Spitzer G, Zander A R, McCredie K B, Dicke K A
Am J Hematol. 1980;9(2):185-92. doi: 10.1002/ajh.2830090206.
To examine the diurnal changes in circulating myeloid progenitor cells (CFU-Cs), 15 normal human volunteers were studied. Total and differential peripheral blood leukocyte counts and CFU-C levels, using the in vitro agar culture method, were obtained at 8 AM, 11 AM, 3 PM, and 8 AM the following morning (in nine subjects) or only at 8 AM and 3 PM (in six subjects). On the average, an approximate twofold rise in circulating CFU C levels (per ml of blood) was found at 3 PM as compared to the 8 AM levels (P 0.002), and a smaller rise at 11 AM was found than at 8 AM levels (P 0.03). The ratios of CFU-C per ml of blood to mononuclear cells per ml of blood and neutrophils per ml of blood, respectively, were significantly higher at 3 PM than at 8 AM (p 0.002 and 0.004, respectively). The rise in CFU-C levels is usually significantly higher when compared to that in peripheral leukocyte counts.
为研究循环髓系祖细胞（集落形成单位 - 粒细胞，CFU - Cs）的昼夜变化，对15名正常人类志愿者进行了研究。采用体外琼脂培养法，于上午8点、上午11点、下午3点以及次日上午8点（9名受试者）或仅于上午8点和下午3点（6名受试者）获取外周血白细胞总数、分类计数及CFU - C水平。平均而言，下午3点时循环CFU - C水平（每毫升血液）相较于上午8点水平升高约两倍（P < 0.002），且上午11点时的升高幅度小于上午8点水平（P < 0.03）。每毫升血液中CFU - C与每毫升血液中单核细胞及中性粒细胞的比例，下午3点时分别显著高于上午8点（分别为P < 0.002和P < 0.004）。与外周血白细胞计数的升高相比，CFU - C水平的升高通常显著更高。
