枯草芽孢杆菌感受态细胞摄取脱氧核糖核酸后裂解活性的增加。
Increase in lytic activity in competent cells of Bacillus subtilis after uptake of deoxyribonucleic acid.
作者信息
Stewart C R, Marmur J
出版信息
J Bacteriol. 1970 Feb;101(2):449-55. doi: 10.1128/jb.101.2.449-455.1970.
Abstract
Extractable lytic activity in competent cells of Bacillus subtilis 168 was markedly increased after treatment with homologous or heterologous deoxyribonucleic acid (DNA). This increase was prevented by deoxyribonuclease, and did not occur with B. subtilis W23 or with noncompetent B. subtilis 168 cells, neither of which take up DNA. Although the deoxyribonuclease-sensitive step in DNA uptake was completed within 10 min, the increase in lytic activity did not begin until more than 30 min after the addition of DNA. The increase was prevented by any of several antibiotics. These results are discussed in relation to the mechanisms for the uptake of transforming DNA and the lysis of transfected cells.
摘要
用同源或异源脱氧核糖核酸（DNA）处理后，枯草芽孢杆菌168感受态细胞中的可提取裂解活性显著增加。脱氧核糖核酸酶可阻止这种增加，而枯草芽孢杆菌W23或无感受态的枯草芽孢杆菌168细胞则不会出现这种情况，这两种细胞均不摄取DNA。尽管DNA摄取过程中对脱氧核糖核酸酶敏感的步骤在10分钟内完成，但裂解活性的增加直到添加DNA后30多分钟才开始。几种抗生素中的任何一种都可阻止这种增加。本文结合转化DNA的摄取机制和转染细胞的裂解对这些结果进行了讨论。
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