Gunson H H, Stratton F, Cooper D G, Rawlinson V I
Br Med J. 1970 Mar 7;1(5696):593-5. doi: 10.1136/bmj.1.5696.593.
To determine the best method for the production of high-titre anti-D serum primary immunization was carried out in two groups of Rh-negative male volunteers with washed group O R(2)R(2) cells. The first group of six men were given 5 ml. of packed cells, and the second group of five men were given 0.5 ml. of packed cells, in each instance by intravenous injection. Only one individual in each group failed to develop anti-D following the primary inoculation, and it has been concluded that 0.5 ml. of packed R(2)R(2) cells is probably a satisfactory dose for this purpose.There was a delay of several weeks before anti-D could be shown to have developed. The initial antibodies which appeared in the serum comprised 7S gammaG immunoglobulins, with, in about half the cases, a minor 19S gammaM component.
为确定生产高效价抗-D血清的最佳方法，对两组Rh阴性男性志愿者用洗涤过的O型R(2)R(2)细胞进行了初次免疫。第一组6名男性静脉注射5毫升压积细胞，第二组5名男性静脉注射0.5毫升压积细胞。每组初次接种后只有1人未产生抗-D，由此得出结论，0.5毫升R(2)R(2)压积细胞可能是达到此目的的合适剂量。抗-D出现前有几周的延迟。血清中最初出现的抗体包括7SγG免疫球蛋白，约半数病例中还有少量19SγM成分。
