Ward M E, Watt P J
J Clin Pathol. 1971 Mar;24(2):122-3. doi: 10.1136/jcp.24.2.122.
Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence. The method described for freezing urethral pus on charcoal swabs should make it possible to transport infected specimens from areas lacking adequate laboratory facilities.
悬浮于含8%甘油的1%蛋白胨中的淋球菌可在液氮中速冻，解冻时无明显损失。这种回收率使得冷冻菌可作为淋球菌大量生长的起始接种物，并能在尿道脓液中以及初次传代培养后保存淋球菌，用于淋球菌毒力研究。所述将尿道脓液冷冻在炭拭子上的方法应能使缺乏足够实验室设施地区的感染标本得以运输。
