Wüst J
J Clin Microbiol. 1977 Dec;6(6):586-90. doi: 10.1128/jcm.6.6.586-590.1977.
By quantitative gas-liquid chromatography of glucose-containing blood cultures at the moment of first signs of growth, a presumptive diagnosis of anaerobic bacteremia could be made in 24 out of 26 cultures yielding obligate anaerobes upon subsequent culture. With Bacteroides sp. (20 strains isolated), elevated levels of isovaleric acid (greater than or equal to 0.1 mumol/ml) and/or succinic acid (greater than or equal to 5 mumol/ml) were detected in the medium used. An exception to this were two strains of Bacteroides thetaiotaomicron that did not produce sufficient quantities of these acids. In the case of butyrate-producing gram-positive cocci (four strains), butyric acid in amounts of greater than or equal to 0.8 mumol/ml was detected. Propionibacteria (five strains) produced propionic acid in amounts of greater than or equal to 3.9 mumol/ml. No false positive results were found in 103 blood cultures with growth of aerobic or facultatively anaerobic bacteria only.
在生长初期迹象出现时，通过对含葡萄糖的血培养物进行定量气液色谱分析，在随后培养出专性厌氧菌的26份培养物中，有24份可做出厌氧菌血症的初步诊断。对于拟杆菌属（分离出20株），在所使用的培养基中检测到异戊酸水平升高（大于或等于0.1μmol/ml）和/或琥珀酸水平升高（大于或等于5μmol/ml）。两株多形拟杆菌是例外，它们产生的这些酸量不足。对于产丁酸革兰氏阳性球菌（4株），检测到丁酸量大于或等于0.8μmol/ml。丙酸杆菌（5株）产生的丙酸量大于或等于3.9μmol/ml。在仅生长需氧菌或兼性厌氧菌的103份血培养物中未发现假阳性结果。
