磷酸三酯谷胱甘肽烷基转移酶。磷酸二甲酯三酯的解毒机制。
Phosphoric acid triester glutathione alkyltransferase. A mechanism for the detoxification of dimethyl phosphate triesters.
作者信息
Hutson D H, Pickering B A, Donninger C
出版信息
Biochem J. 1972 Mar;127(1):285-93. doi: 10.1042/bj1270285.
Abstract
- 2-Chloro-1-(2,4,5-trichlorophenyl)vinyl dimethyl phosphate (tetrachlorvinphos) is demethylated by mammalian liver supernatant (100000g) protein in the presence of GSH. 2. GSH acts as an acceptor of the transferred methyl group to form S-methyl glutathione. 3. The enzyme that catalyses this reaction is present in the soluble fraction of liver from mouse, rat, rabbit and pig at similar activity. The enzyme was purified 45-fold from pig liver, dimethyl 1-naphthyl phosphate being used as assay substrate. 4. Methyl groups are readily removed from most of the substrates studied; ethyl groups are removed at one-fiftieth to one-hundredth of the rate for methyl groups. It is likely that the enzyme plays an important role in the detoxification of the phosphate triester pesticides containing CH(3)-O-P groups.
摘要
- 2-氯-1-(2,4,5-三氯苯基)乙烯基二甲基磷酸酯（毒死蜱）在谷胱甘肽存在的情况下，被哺乳动物肝脏上清液（100000g）蛋白脱甲基。2. 谷胱甘肽作为转移甲基的受体，形成S-甲基谷胱甘肽。3. 催化此反应的酶存在于小鼠、大鼠、兔子和猪肝脏的可溶性部分，活性相似。该酶从猪肝中纯化了45倍，使用磷酸二甲酯-1-萘酯作为测定底物。4. 甲基很容易从大多数研究的底物中去除；乙基的去除速率是甲基的五十分之一到百分之一。该酶可能在含有CH(3)-O-P基团的磷酸三酯农药解毒中起重要作用。
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