重症肌无力中抗烟碱型乙酰胆碱受体抗体的改良检测方法。
A modified assay for antibody against the nicotinic acetylcholine receptor in myasthenia gravis.
作者信息
Dwyer D S, Bradley R J, Oh S J, Kemp G E
出版信息
Clin Exp Immunol. 1979 Sep;37(3):448-51.
Myasthenia gravis is an autoimmune disease which may be detected by the presence of serum antibodies against the nicotinic acetylcholine receptor at the neuromuscular junction. Immunoprecipitation assays have been developed to measure these immunoglobulins and calculate titres. These assays require the labelling of the receptor with 125I-alpha-bungarotoxin which binds irreversibly. However, the standard immunoprecipitation assay may significantly underestimate the titres of some myasthenic patients. We have discovered patients with antibodies specific for the alpha-bungarotoxin binding site of purified rat muscle receptor. If labelled toxin is already present on the receptor, these antibodies are unable to bind to the protein. This phenomenon may lead to underestimates of the actual antibody titre. To circumvent this problem, we have designed a modified immunoprecipitation assay to evaluate titres.
重症肌无力是一种自身免疫性疾病，可通过血清中存在针对神经肌肉接头处烟碱型乙酰胆碱受体的抗体来检测。已经开发出免疫沉淀测定法来测量这些免疫球蛋白并计算滴度。这些测定法需要用不可逆结合的125I-α-银环蛇毒素标记受体。然而，标准免疫沉淀测定法可能会显著低估一些重症肌无力患者的滴度。我们发现了一些患者，他们的抗体对纯化大鼠肌肉受体的α-银环蛇毒素结合位点具有特异性。如果标记毒素已经存在于受体上，这些抗体就无法与该蛋白质结合。这种现象可能导致对实际抗体滴度的低估。为了解决这个问题，我们设计了一种改良的免疫沉淀测定法来评估滴度。
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