Biendo M, Eb F, Lefebvre J F, Orfila J
Laboratoire de Bactériologie-Immunologie Générale, CHU Nord, France.
Clin Diagn Lab Immunol. 1996 Nov;3(6):706-9. doi: 10.1128/cdli.3.6.706-709.1996.
The Western blot (immunoblot) patterns of 56 serum specimens, all examined previously by the microimmunofluorescence (MIF) test for species-specific Chlamydia antibodies, were analyzed. Predominant specific-antibody activity was directed to the 170-, 155-, 145-, 120-, 115-, 100-, 57-, and 38-kDa proteins of Chlamydia trachomatis and to the 175-, 130-, 110-, 98-, and 30-kDa proteins of Chlamydia pneumoniae. All of these antigens appeared to be species specific. The reactivity with 90-, 80-, 75-, 62- or 60-, and 55-kDa proteins and the major outer membrane protein appeared to be genus specific. Fourteen serum samples which had identical titers of immunoglobulin G as determined by the MIF test were investigated by Western blotting. We found that nine serum samples had anti-C. trachomatis protein profiles and two had anti-C. pneumoniae protein profiles. The double seropositivity observed by MIF corresponded with cross-reactivity to genus-specific antibodies. As for the three remaining serum specimens, we observed identical protein profiles for C. trachomatis and C. pneumoniae, confirming the double seropositivity experienced with the MIF test. Western blotting can differentiate between specific reactions and interfering noise from other, partly cross-reacting chlamydial species.
分析了56份血清标本的蛋白质印迹（免疫印迹）图谱，所有标本之前均通过微量免疫荧光（MIF）试验检测了沙眼衣原体属特异性抗体。主要的特异性抗体活性针对沙眼衣原体的170、155、145、120、115、100、57和38 kDa蛋白，以及肺炎衣原体的175、130、110、98和30 kDa蛋白。所有这些抗原似乎都是种特异性的。与90、80、75、62或60以及55 kDa蛋白和主要外膜蛋白的反应性似乎是属特异性的。通过蛋白质印迹法研究了14份经MIF试验测定免疫球蛋白G滴度相同的血清样本。我们发现，9份血清样本具有抗沙眼衣原体蛋白谱，2份具有抗肺炎衣原体蛋白谱。MIF观察到的双重血清阳性与对属特异性抗体的交叉反应相对应。至于其余3份血清标本，我们观察到沙眼衣原体和肺炎衣原体具有相同的蛋白谱，证实了MIF试验出现的双重血清阳性。蛋白质印迹法可以区分特异性反应和来自其他部分交叉反应衣原体种的干扰性噪声。
