从产头孢菌素C的头孢霉属菌株中分离细胞内肽段及其性质
Isolation and nature of intracellular peptides from a cephalosporin C-producing Cephalosporium sp.
作者信息
Loder P B, Abraham E P
出版信息
Biochem J. 1971 Jul;123(3):471-6. doi: 10.1042/bj1230471.
Abstract
- Three peptides containing alpha-aminoadipic acid and cysteine have been obtained in small amounts from the mycelium of a Cephalosporium sp. 2. The peptides were precipitated as cuprous mercaptides together with glutathione and resolved from the latter and from each other by preparative paper electrophoresis and chromatography either in the sulphonic acid form or as S-sulphonyl derivatives. From the S-sulphonyl derivatives they were obtained in the thiol form. 3. One peptide (P3) was shown by amino acid analysis and the mass spectrum of the NS-ethoxycarbonyl derivative of its methyl ester to be delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine. A second peptide (P2) contained alpha-aminoadipic acid, cysteine, valine and glycine, and the third peptide (P1) contained alpha-aminoadipic acid, cysteine, beta-hydroxyvaline and glycine.
摘要
- 从一种头孢霉属真菌的菌丝体中少量获得了三种含α-氨基己二酸和半胱氨酸的肽。2. 这些肽与谷胱甘肽一起以亚铜硫醇盐的形式沉淀，并通过制备性纸电泳和色谱法从后者以及彼此中分离出来，分离形式为磺酸形式或S-磺酰基衍生物。从S-磺酰基衍生物中，它们以硫醇形式获得。3. 通过氨基酸分析及其甲酯的N-乙氧羰基衍生物的质谱表明，一种肽（P3）为δ-(l-α-氨基己二酰)-l-半胱氨酰-d-缬氨酸。第二种肽（P2）含有α-氨基己二酸、半胱氨酸、缬氨酸和甘氨酸，第三种肽（P1）含有α-氨基己二酸、半胱氨酸、β-羟基缬氨酸和甘氨酸。
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本文引用的文献
1
The cuprous derivatives of some sulphydryl compounds.某些巯基化合物的亚铜衍生物。
Biochem J. 1931;25(2):614-28. doi: 10.1042/bj0250614.
2
Sulphur-containing amino acids and peptides in the mycelium of Penicillium chrysogenum.产黄青霉菌丝体中的含硫氨基酸和肽。
Biochem J. 1960 Aug;76(2):353-7. doi: 10.1042/bj0760353.
3
Studies on the reaction of sulfite with proteins.亚硫酸盐与蛋白质反应的研究。
J Biol Chem. 1959 Jul;234(7):1733-9.
4
A modified ninhydrin reagent for the photometric determination of amino acids and related compounds.一种用于光度法测定氨基酸及相关化合物的改良茚三酮试剂。
J Biol Chem. 1954 Dec;211(2):907-13.
5
The role of valine in the biosynthesis of penicillin N and cephalosporin C by a Cephalosporium sp.缬氨酸在头孢霉属某菌株合成青霉素N和头孢菌素C中的作用
Biochem J. 1967 Jun;103(3):902-12. doi: 10.1042/bj1030902.
6
Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of Cephalosporium sp.青霉素N和头孢菌素C的生物合成。头孢霉属的抗生素生产及代谢的其他特征
Biochem J. 1967 Jun;103(3):877-90. doi: 10.1042/bj1030877.
7
Behaviour of alpha-aminoadipylcysteine and glutamylcysteine in the presence of intact and disrupted mycelium of a Cephalosporium sp.α-氨基己二酰半胱氨酸和谷氨酰半胱氨酸在完整及破碎的头孢霉属真菌菌丝体存在情况下的行为
Biochem J. 1969 May;112(4):389-96. doi: 10.1042/bj1120389.
8
Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis.从枯草芽孢杆菌培养滤液中分离杆菌溶素和一种新氨基酸。
Biochem J. 1970 Jul;118(4):557-61. doi: 10.1042/bj1180557.
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