从枯草芽孢杆菌培养滤液中分离杆菌溶素和一种新氨基酸。
Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis.
作者信息
Walker J E, Abraham E P
出版信息
Biochem J. 1970 Jul;118(4):557-61. doi: 10.1042/bj1180557.
Abstract
- Bacilysin, a labile dipeptide antibiotic that lyses growing staphylococci, was isolated from culture fluids of Bacillus subtilis by a process giving higher yields than those previously obtained. 2. The process involves adsorption on a cation-exchange resin and elution with aqueous trimethylamine, separation from neutral amino acids and glutamic acid by chromatography on DEAE-Sephadex at pH8.7 and separation from other neutral peptides by chromatography in aqueous propan-2-ol on Sephadex G-25. 3. A new amino acid, which is chemically related to bacilysin, was isolated from the fraction containing neutral amino acids. 4. Two substances that yield alanine on hydrolysis, in addition to bacilysin, were obtained from the neutral peptide fraction.
摘要
- 杆菌溶素是一种能溶解生长中的葡萄球菌的不稳定二肽抗生素，它是通过一种比以前获得的产量更高的方法从枯草芽孢杆菌的培养液中分离出来的。2. 该方法包括吸附在阳离子交换树脂上，并用三甲胺水溶液洗脱，在pH8.7条件下通过DEAE-葡聚糖凝胶柱色谱从中性氨基酸和谷氨酸中分离，以及在Sephadex G-25上用2-丙醇水溶液柱色谱从其他中性肽中分离。3. 从含有中性氨基酸的馏分中分离出一种与杆菌溶素化学相关的新氨基酸。4. 除杆菌溶素外，从中性肽馏分中还获得了两种水解时产生丙氨酸的物质。
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Biochem J. 1970 Jul;118(4):563-70. doi: 10.1042/bj1180563.
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