核小体在浓盐溶液中的解离与转移
Nucleosome dissociation and transfer in concentrated salt solutions.
作者信息
Stacks P C, Schumaker V N
出版信息
Nucleic Acids Res. 1979 Dec 20;7(8):2457-67. doi: 10.1093/nar/7.8.2457.
Abstract
We have examined the dissociation of nucleosomes into histones and free, 4.5S DNA over a range of sodium chloride concentrations between 0.25 and 1 M. We have also studied this dissociation as a function of nucleosome concentration at two salt concentrations, 0.8 M and 0.9 M. In addition, we have measured the kinetics of transfer of histone cores from nucleosomes onto recipient bacteriophage T7 DNA in 0.6, 0.7 and 0.8 M NaCl solutions. Although the mechanism of nucleosome transfer is unknown the data presented here are consistent with either a reversible dissociation of the nucleosome or DNA strand displacement by another DNA.
摘要
我们研究了在0.25至1M的一系列氯化钠浓度范围内，核小体解离为组蛋白和游离的4.5S DNA的情况。我们还研究了在0.8M和0.9M两种盐浓度下，这种解离与核小体浓度的关系。此外，我们测量了在0.6M、0.7M和0.8M NaCl溶液中，组蛋白核心从核小体转移到受体噬菌体T7 DNA上的动力学。尽管核小体转移的机制尚不清楚，但此处呈现的数据与核小体的可逆解离或另一条DNA的DNA链置换是一致的。
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