Uemura T, Sakaguchi G, Ito T, Okazawa K, Sakai S
Jpn J Med Sci Biol. 1975 Jun;28(3):165-77. doi: 10.7883/yoken1952.28.165.
Purified C. perfringens type A enterotoxin fed orally in an amount of 5 mg caused both vomiting and diarrhea in the monkey only when the gastric juice had been neutralized. Exposure of enterotoxin to pH 4.0 or below rapidly destroyed the activity. All three monkeys receiving sodium bicarbonate and 2.4 X 10(10) viable cells grown in DS medium developed diarrhea, and only one of them vomited once. The diarrhea lasted for 13, 18 and 19 hr. The symptoms were similar to those reported in human cases of C. perfringens food poisoning. These results have verified the general notion that C. perfringens food poisoning should be categorized as a true "intravital intoxication". The reversed passive hemagglutination test detected enterotoxin directly in most fecal samples. This method may be applicable for diagnosis of human cases of C. perfringens food poisoning. Neither enterotoxin nor anti-enterotoxin was detected in serum samples taken from any monkey up to 21 days after the challenge. We are tempted to conclude, therefore, that no significant amount of C. perfringens enterotoxin is absorbed from the intestine.
仅在胃液被中和时，经口投喂5毫克纯化的A型产气荚膜梭菌肠毒素才会使猴子出现呕吐和腹泻症状。肠毒素暴露于pH 4.0或更低的环境中会迅速丧失活性。所有三只接受碳酸氢钠和在DS培养基中培养的2.4×10(10)个活细胞的猴子都出现了腹泻，其中只有一只呕吐了一次。腹泻持续了13、18和19小时。这些症状与人类产气荚膜梭菌食物中毒病例中报告的症状相似。这些结果证实了产气荚膜梭菌食物中毒应归类为真正的“活体中毒”这一普遍观点。反向被动血凝试验在大多数粪便样本中直接检测到了肠毒素。该方法可能适用于人类产气荚膜梭菌食物中毒病例的诊断。在攻击后长达21天的时间里，从任何猴子采集的血清样本中均未检测到肠毒素或抗肠毒素。因此，我们倾向于得出结论，即没有大量的产气荚膜梭菌肠毒素从肠道吸收。
