减毒流感病毒的比较研究
A comparative study of attenuated influenza viruses.
作者信息
Beare A S, Maassab H F, Tyrrell D A, Slepuskin A N, Hall T S
出版信息
Bull World Health Organ. 1971;44(5):593-8.
Abstract
Influenza A and influenza B viruses were adapted to growth at 25 degrees C. When given to volunteer subjects, the viruses were attenuated but remained infective and antigenic. The minimum immunizing dose of an egg-adapted virus appeared to be 10(5.0) EID(50). Cloning by plaque selection at 25 degrees C gave seed cultures of relatively low infectivity titres. These titres were increased when necessary by passage at 33 degrees C. No reversion to virulence was observed.Viruses attenuated in the United Kingdom and the USA were compared in volunteer trials with vaccine strains that had already been used in the USSR for mass immunization. Results were broadly similar. Currently available methods of attenuation and work with temperature-sensitive mutants are reviewed.
摘要
甲型和乙型流感病毒被适应于在25摄氏度下生长。当给予志愿者受试者时，病毒被减毒但仍具有感染性和抗原性。适应鸡蛋的病毒的最小免疫剂量似乎是10(5.0) EID(50)。在25摄氏度下通过噬斑选择进行克隆得到了感染性滴度相对较低的种子培养物。必要时通过在33摄氏度下传代可提高这些滴度。未观察到毒力回复。在志愿者试验中，将在英国和美国减毒的病毒与苏联已用于大规模免疫的疫苗株进行了比较。结果大致相似。综述了目前可用的减毒方法以及对温度敏感突变体的研究。
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