大肠杆菌中甘油激酶结构基因的染色体定位。
Chromosomal location of the structural gene for glycerol kinase in Escherichia coli.
作者信息
Cozzarelli N R, Lin E C
出版信息
J Bacteriol. 1966 May;91(5):1763-6. doi: 10.1128/jb.91.5.1763-1766.1966.
Abstract
Cozzarelli, N. R. (Harvard Medical School, Boston, Mass.), and E. C. C. Lin. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J. Bacteriol. 91:1763-1766. 1966.-A glycerol kinase mutant site has been mapped by transduction and sexual conjugation. Three-factor crosses with the two procedures yielded the following gene order: arginine-1-methionine-1-glycerol kinase-isoleucine, valine-16. An additional 13 independent glycerol kinase mutant sites mapped in the same region. Since some of the mutants were able to produce a protein serologically indistinguishable from the wild-type enzyme, it is concluded that the region mapped represents the structural gene for the kinase.
摘要
科扎雷利，N.R.（哈佛医学院，马萨诸塞州波士顿）和E.C.C.林。大肠杆菌中甘油激酶结构基因的染色体定位。《细菌学杂志》91:1763 - 1766。1966年。- 通过转导和有性接合对一个甘油激酶突变位点进行了定位。采用这两种方法进行的三因子杂交产生了以下基因顺序：精氨酸 - 1 - 蛋氨酸 - 1 - 甘油激酶 - 异亮氨酸，缬氨酸 - 16。另外13个独立的甘油激酶突变位点定位于同一区域。由于一些突变体能够产生在血清学上与野生型酶无法区分的蛋白质，因此得出结论，所定位的区域代表激酶的结构基因。
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