Thompson H C, Eisenstein T K
Infect Immun. 1976 Mar;13(3):750-7. doi: 10.1128/iai.13.3.750-757.1976.
A subcellular fraction, designated PSP-3R, prepared from rough, type 3R Streptococcus pneumoniae is described, which affords excellent protection to mice against challenge with smooth organisms of the homologous serotype 3S and significant protection against heterologous challenge with serotypes 1S and 2S. Adjuvant enhances the protective capacity of the vaccine but is not necessary for immunogenicity. Protection induced by PSP-3R can be passively transferred tp normal mice with serum from actively immunized animals. The protective capacity can be completely absorbed out with rough or smooth type 3 organisms but not with rough type 1R or 2R cells. PSP-3R immune serum was tested in a passive hemagglutination assay against type 3 capsular polysaccharide-coated erythrocytes and found to have no detectable anticapsular antibody. The possible identity of the immunogen (s) in the vaccine is discussed.
本文描述了一种从粗糙的3R型肺炎链球菌制备的亚细胞组分，命名为PSP-3R，它能为小鼠提供极好的保护，使其免受同源3S型光滑菌的攻击，并对1S和2S型异源攻击提供显著保护。佐剂可增强疫苗的保护能力，但对免疫原性并非必需。PSP-3R诱导的保护作用可通过主动免疫动物的血清被动转移至正常小鼠。保护能力可被粗糙或光滑的3型菌完全吸收，但不能被粗糙的1R或2R型细胞吸收。在针对3型荚膜多糖包被红细胞的被动血凝试验中检测了PSP-3R免疫血清，发现其无可检测到的抗荚膜抗体。文中还讨论了疫苗中免疫原的可能身份。
