Eichmann K, Braun D G, Feizi T, Krause R M
J Exp Med. 1970 Jun 1;131(6):1169-89. doi: 10.1084/jem.131.6.1169.
Electrophoretically monodisperse antibody components in rabbit antisera to the carbohydrates of the Groups A and C streptococci have been examined for their individual antigenic specificity. In these antibody components which were isolated by preparative electrophoresis, individual antigenic specificity was confined to the specific antibody and was absent in the nonantibody gamma-globulin. Radioprecipitation experiments and the use of immune absorbent columns constructed from goat anti-antisera, which had been absorbed with fraction II, revealed that all the specific antibody in an electrophoretically monodisperse component was reactive with the homologous anti-antibody. Antibodies with either identical or distinct individual antigenic specificities may occur in the same rabbit with repeated immunizations. Antibodies with identical antigenic specificity had identical electrophoretic mobility, whereas antibodies with unrelated antigenic specificities had distinct electrophoretic mobilities. In the interval between immunizations, if antibody to the carbohydrate antigen was absent, there was no detectable antibody with individual antigenic specificity.
对兔抗A组和C组链球菌碳水化合物抗血清中经电泳呈现单分散的抗体成分进行了个体抗原特异性检测。在这些通过制备性电泳分离得到的抗体成分中，个体抗原特异性局限于特异性抗体，而非抗体γ球蛋白中不存在该特异性。放射沉淀实验以及使用由用II组分吸收过的山羊抗抗血清构建的免疫吸附柱表明，电泳单分散成分中的所有特异性抗体都能与同源抗抗体发生反应。重复免疫时，具有相同或不同个体抗原特异性的抗体可能出现在同一只兔子体内。具有相同抗原特异性的抗体具有相同的电泳迁移率，而具有不相关抗原特异性的抗体具有不同的电泳迁移率。在免疫间隔期，如果不存在针对碳水化合物抗原的抗体，则检测不到具有个体抗原特异性的抗体。
