Galey W R, Van Bruggen J T
J Gen Physiol. 1970 Feb;55(2):220-42. doi: 10.1085/jgp.55.2.220.
Our previous description of solute drag on a synthetic membrane has been extended to include the solutes mannitol, sucrose, raffinose, inulin, and dextran. Labeled and nonlabeled forms of these solutes were used in pairs to quantitate solute flux interaction. Three membranes with pore sizes of 350, 80, and 20 A, respectively, have been utilized. It is shown that solute flux interaction occurs with all the solutes and that the extent of interaction is related directly to solute permeability, concentration, and molecular size. The magnitude of solute interaction is reciprocally related to the radii of the membrane pores, greater interaction occurring with small pored membranes. Solute drag is seen as an increased flux of tracer solute in the direction of the diffusion gradient of a second solute as well as a decreased tracer flux into the diffusion gradient. Values are given for self-diffusion and interaction coefficients as well as for a new coefficient, the "effectiveness coefficient."
我们之前对合成膜上溶质拖曳的描述已扩展至包括甘露醇、蔗糖、棉子糖、菊粉和右旋糖酐等溶质。这些溶质的标记形式和未标记形式成对使用，以定量溶质通量相互作用。分别使用了孔径为350埃、80埃和20埃的三种膜。结果表明，所有溶质都会发生溶质通量相互作用，且相互作用的程度与溶质渗透率、浓度和分子大小直接相关。溶质相互作用的大小与膜孔半径成反比，小孔径膜的相互作用更大。溶质拖曳表现为示踪溶质在第二种溶质扩散梯度方向上通量增加，以及进入扩散梯度的示踪通量减少。给出了自扩散系数、相互作用系数以及一个新系数“有效系数”的值。
