Brown N D, Sweet R B, Kintzios J A, Cox H D, Doctor B P
J Chromatogr. 1979 Sep 11;164(1):35-40. doi: 10.1016/s0378-4347(00)81569-8.
A sensitive and specific method for the determination of diamines and polyamines by ion-pair high-performance liquid chromatography is described. The 5-dimethylaminonaphthalene-1-sulfonyl derivatives of putrescine, 1,6-diaminohexane, spermidine and spermine are separated on a muBondapak C18 reversed-phase column with 1-heptanesulfonic acid and acetonitrile as the mobile phase. All compounds are eluted within 30 min using a programmed solvent gradient system. The method has a lower detection limit of 1 pmole on column. Because of the simplicity of the method, its application provides a better means for closely monitoring patients undergoing treatment for various types of genito-urinary neoplastic diseases.
本文描述了一种通过离子对高效液相色谱法测定二胺和多胺的灵敏且特异的方法。腐胺、1,6 - 己二胺、亚精胺和精胺的5 - 二甲基氨基萘 - 1 - 磺酰衍生物在μBondapak C18反相柱上进行分离，流动相为1 - 庚烷磺酸和乙腈。使用程序溶剂梯度系统，所有化合物在30分钟内洗脱完毕。该方法柱上检测限低至1皮摩尔。由于该方法操作简便，其应用为密切监测各类泌尿生殖系统肿瘤疾病患者的治疗情况提供了更好的手段。
