Dusheĭko A A, Chernukhina L A, Blazhevich M A
Ukr Biokhim Zh (1978). 1979 Nov-Dec;51(6):653-8.
The lipid composition of secretion and the vitamin A content in it were studied. Secretion of normal chickens is shown to contain 96 +/- 3.1 mg of lipids, 82.5 +/- 3.4 of substances extracted by the solvent hexane-diethyl ester, 30 +/- 2.1 of unsaponifiable lipids and 2.43 +/- 0.285 micrograms of vitamin A per 1 g of dry residue. With A-avitaminosis the secretion amount is twice as large with vitamin A absent; the lipid content is almost twice as low, the total weight of substances extracted by the solvent hexane-diethyl ester is four times as low and that of the lipid unsaponifiable fraction--2.5 times as low. Quantitative and qualitative changes in lipid zones were detected by means of thin-layer chromatography and UV-spectroscopy, and structural changes in the secretion--by electron microscopy. Vitamin A is suggested to participate in formation of macromolecular lipid systems of the secretion.
对分泌物的脂质组成及其维生素A含量进行了研究。结果表明，正常鸡的分泌物每1克干残渣中含有96±3.1毫克脂质、82.5±3.4毫克可被己烷 - 二乙酯溶剂萃取的物质、30±2.1毫克不皂化脂质以及2.43±0.285微克维生素A。在维生素A缺乏症的情况下，分泌物量在缺乏维生素A时是正常量的两倍；脂质含量几乎低了一半，可被己烷 - 二乙酯溶剂萃取的物质总重量低了四倍，而脂质不皂化部分低了2.5倍。通过薄层色谱法和紫外光谱法检测了脂质区的定量和定性变化，通过电子显微镜检测了分泌物的结构变化。提示维生素A参与分泌物大分子脂质系统的形成。
