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接触敏感性中的淋巴细胞转化

Lymphocyte transformation in contact sensitivity.

作者信息

Geczy A F, Baumgarten A

出版信息

Immunology. 1970 Aug;19(2):189-203.

PMID:5470192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1455749/
Abstract

Leucocyte suspensions containing 97–100 per cent lymphocytes were prepared from the peripheral blood of unsensitized guinea-pigs as well as animals previously sensitized with 1-chloro-2,4-dinitrobenzene. The lymphocytes were dinitrophenylated and cultured for 5 days . Transformed cells (8–22 per cent) and an increased (100–1000 fold) uptake of tritiated thymidine were observed only in cultures of lymphocytes from sensitized animals. Smaller numbers of transformed cells (1–2 per cent) were found in only a few cultures of sensitive lymphocytes incubated with either dinitrophenylated autologous erythrocytes or serum proteins. These results strongly suggest that transformation may specifically follow the conjugation of DNFB with live lymphocytes from sensitized donors.

摘要

从未致敏的豚鼠以及先前用1-氯-2,4-二硝基苯致敏的动物的外周血中制备了含有97%-100%淋巴细胞的白细胞悬液。将淋巴细胞二硝基苯基化并培养5天。仅在致敏动物的淋巴细胞培养物中观察到转化细胞（8%-22%）以及氚标记胸腺嘧啶核苷摄取增加（100-1000倍）。在用二硝基苯基化的自体红细胞或血清蛋白孵育的敏感淋巴细胞的少数培养物中，发现了较少数量的转化细胞（1%-2%）。这些结果有力地表明，转化可能特异性地跟随二硝基氟苯与致敏供体的活淋巴细胞的结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/2c4a462db1f0/immunology00367-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/272bbfae18a0/immunology00367-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/0c24204a6e88/immunology00367-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/d3eebd1ae837/immunology00367-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/ce506eb58c64/immunology00367-0016-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/2c4a462db1f0/immunology00367-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/272bbfae18a0/immunology00367-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/0c24204a6e88/immunology00367-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/d3eebd1ae837/immunology00367-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/ce506eb58c64/immunology00367-0016-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7399/1455749/2c4a462db1f0/immunology00367-0017-a.jpg

相似文献

1
Lymphocyte transformation in contact sensitivity.接触敏感性中的淋巴细胞转化
Immunology. 1970 Aug;19(2):189-203.
2
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4
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Lymphocyte transformation in contact sensitivity.接触敏感性中的淋巴细胞转化
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The mononuclear-cell infiltrate in allergic contact dermatitis. 1. Participation of thymic cells.变应性接触性皮炎中的单核细胞浸润。1. 胸腺细胞的参与。
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In vitro lymphocyte responses in contact hypersensitivity IV.接触性超敏反应中的体外淋巴细胞反应IV。
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The role of lipid in the induction of hapten-specific delayed hypersensitivity and contact sensitivity.脂质在诱导半抗原特异性迟发型超敏反应和接触性过敏反应中的作用。
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Cell proliferation in epidermis during the development of contact reactions as revealed by autoradiography after injection of thymidine-H3. 2. The lymphoid cells.注射氚标记胸腺嘧啶核苷后通过放射自显影术揭示的接触反应发展过程中表皮中的细胞增殖。2. 淋巴细胞。
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引用本文的文献

1
Hapten-cell conjugates in DNCB contact sensitivity. In vitro stimulation with DNP-conjugates optimally inducing contact sensitivity in vivo.二硝基氯苯接触敏感性中的半抗原-细胞缀合物。用二硝基苯基缀合物进行体外刺激可在体内最佳地诱导接触敏感性。
Immunology. 1980 Feb;39(2):291-9.
2
Allergic contact dermatitis to methylenelactones. Use of lymphocyte transformation test.对亚甲基内酯的过敏性接触性皮炎。淋巴细胞转化试验的应用。
Arch Dermatol Res. 1982;272(1-2):73-8. doi: 10.1007/BF00510396.
3
Induction of leucocyte migration inhibitory factor (LIF) by stimulation with free hapten and water-insoluble epoxy resin.

本文引用的文献

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Elicitation of delayed allergic skin reactions with haptens; the dependence of elicitation on hapten combination with protein.用半抗原引发迟发性过敏性皮肤反应；引发反应对半抗原与蛋白质结合的依赖性。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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THE ROLE OF LYMPHOCYTES IN THE SENSITIZATION OF RATS TO RENAL HOMOGRAFTS.淋巴细胞在大鼠对肾同种异体移植致敏中的作用。
通过游离半抗原和水不溶性环氧树脂刺激诱导白细胞迁移抑制因子（LIF）
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Induction of delayed hypersensitivity by dinitrophenylated lymphocytes.二硝基苯基化淋巴细胞诱导迟发型超敏反应。
Immunology. 1970 Aug;19(2):205-17.
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Distribution of a contact sensitizer, 1-fluoro-2,4-dinitro-benzene, in the the tissues of the guinea-pig.接触性致敏剂1-氟-2,4-二硝基苯在豚鼠组织中的分布。
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Lymphocyte transformation during dinitrochlorobenzene contact sensitization. An in vitro and in vivo evaluation of the primary immune response in man.二硝基氯苯接触致敏期间的淋巴细胞转化。人体初次免疫反应的体外和体内评估。
J Clin Invest. 1973 Aug;52(8):1925-30. doi: 10.1172/JCI107376.
7
Contact sensitivity in vitro. The production of macrophage inhibition factors from DNCB sensitized lymphocytes by subcellular organelles obtained from DNCB epidermal tissue.体外接触敏感性。从二硝基氯苯（DNCB）致敏淋巴细胞中由DNCB表皮组织获得的亚细胞器产生巨噬细胞抑制因子。
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8
Increased DNA synthesis in vitro in guinea-pigs unresponsive to DNP--skin protein conjugate.对二硝基苯酚-皮肤蛋白结合物无反应的豚鼠体外DNA合成增加。
Immunology. 1974 Jul;27(1):115-24.
9
Lymphocyte transformation to membrane-conjugated, liposome-conjugated, or unconjugated pentadecylcatechol in the guinea pig.豚鼠中淋巴细胞向与膜结合、与脂质体结合或未结合的十五烷基儿茶酚的转化。
Arch Dermatol Res. 1985;277(3):209-13. doi: 10.1007/BF00404318.
10
Hapten-specific lymphocyte transformation in humans sensitized with NDMA or DNCB.用N-亚硝基二甲胺（NDMA）或二硝基氯苯（DNCB）致敏的人体中的半抗原特异性淋巴细胞转化
Clin Exp Immunol. 1976 Sep;25(3):490-2.
J Exp Med. 1965 Aug 1;122(2):347-60. doi: 10.1084/jem.122.2.347.
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DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS.体外迟发型超敏反应。I. 抗原对细胞迁移抑制作用的特异性。
J Immunol. 1964 Aug;93:264-73.
5
A vascular permeability factor extracted from normal and sensitized guinea-pig lymph node cells.从正常和致敏豚鼠淋巴结细胞中提取的一种血管通透性因子。
Life Sci (1962). 1962 Jul;1:347-52. doi: 10.1016/0024-3205(62)90131-5.
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Tuberculin-induced mitosis in peripheral blood leucocytes.结核菌素诱导外周血白细胞有丝分裂。
Lancet. 1963 Mar 23;1(7282):637-8. doi: 10.1016/s0140-6736(63)91275-3.
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The homograft reaction.同种移植反应。
Proc R Soc Lond B Biol Sci. 1958 Dec 4;149(935):145-66. doi: 10.1098/rspb.1958.0058.
8
Elicitation of allergic contact dermatitis in the guinea pig; the distribution of bound dinitrobenzene groups within the skin and quantitative determination of the extent of combination of 2,4-dinitro-chlorobenzene with epidermal protein in vivo.豚鼠过敏性接触性皮炎的诱发；皮肤内结合二硝基苯基团的分布以及体内2,4-二硝基氯苯与表皮蛋白结合程度的定量测定。
J Exp Med. 1958 Dec 1;108(6):773-96. doi: 10.1084/jem.108.6.773.
9
Determination of cell viability.细胞活力的测定。
Proc Soc Exp Biol Med. 1958 May;98(1):188-92. doi: 10.3181/00379727-98-23985.
10
Response of human blood lymphocytes to tuberculin PPD in tissue culture.人血淋巴细胞在组织培养中对结核菌素纯蛋白衍生物的反应。
Immunology. 1967 Apr;12(4):417-29.