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通过游离半抗原和水不溶性环氧树脂刺激诱导白细胞迁移抑制因子（LIF）

Induction of leucocyte migration inhibitory factor (LIF) by stimulation with free hapten and water-insoluble epoxy resin.

作者信息

Nakano Y, Nakano K, Hara I

出版信息

Clin Exp Immunol. 1981 Aug;45(2):419-26.

PMID:7032773
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1537392/
Abstract

The indirect leucocyte migration inhibition test was applied to the contact sensitivity to a hapten (dinitrofluorobenzene, DNFB) and a water-insoluble epoxy resin (Araldite). Sensitized guinea-pig leucocytes were pulsed with DNFB, which was not conjugated to a carrier protein, or with Araldite which was made particulate by ultrasonication. When these pulsed leucocytes were incubated for 24 hr, they released significant amounts of leucocyte migration inhibitory factor (LIF). The optimal dose for pulse-stimulation was 10 microgram/ml. A correlation was observed between the degree of contact sensitivity and the production of LIF.

摘要

间接白细胞迁移抑制试验应用于对半抗原（二硝基氟苯，DNFB）和水不溶性环氧树脂（Araldite）的接触敏感性研究。用未与载体蛋白结合的DNFB或经超声处理制成颗粒状的Araldite刺激致敏豚鼠白细胞。当这些经刺激的白细胞孵育24小时后，它们释放出大量的白细胞迁移抑制因子（LIF）。脉冲刺激的最佳剂量为10微克/毫升。观察到接触敏感性程度与LIF产生之间存在相关性。

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