快速检测干制食品中肠杆菌科亚致死损伤细胞
Rapid detection of sublethally impaired cells of Enterobacteriaceae in dried foods.
作者信息
Mossel D A, Ratto M A
出版信息
Appl Microbiol. 1970 Aug;20(2):273-5. doi: 10.1128/am.20.2.273-275.1970.
Abstract
Samples of dried foods, feeds, and drugs were examined for Enterobacteriaceae by using buffered glucose-Brilliant Green-bile broth as the final enrichment medium and deep tubes of violet-red-bile-glucose-agar for confirmation. The pre-enrichment treatments used for the resuscitation of sublethally impaired cells were overnight incubation in lactose broth versus incubation for 1 to 6 hr at room temperature in shallow layers of tryptone soya peptone broth. The latter restoration treatment appeared to be significantly more productive.
摘要
采用缓冲葡萄糖-煌绿胆盐肉汤作为最终增菌培养基，并用紫红胆盐葡萄糖琼脂深层试管进行确证，对干制食品、饲料和药物样本进行了肠杆菌科检测。用于亚致死损伤细胞复苏的预增菌处理方法有两种，一种是在乳糖肉汤中过夜培养，另一种是在胰蛋白胨大豆蛋白胨肉汤浅层于室温下培养1至6小时。后一种复苏处理方法的效果似乎明显更好。
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