Davis N D, Diener U L, Morgan-Jones G
Appl Environ Microbiol. 1977 Aug;34(2):155-7. doi: 10.1128/aem.34.2.155-157.1977.
Cultures of Alternaria alternata (three isolates) and Alternaria tenuissima (three isolates) obtained from cottonseeds and bolls were toxigenic when cultured on various laboratory media. A mycotoxin was isolated and identified as tenuazonic acid by using solvent partition, thin-layer chromatography, and instrument analyses. Toxicity was monitored with brine shrimp and chicken embryo bioassays. All cultures except A. alternata 938 produced tenuazonic acid when grown on cottonseed and on yeast extract-sucrose broth. The most toxin (266 mg/kg) was produced by A. tenuissima 843 on cottonseed.
从棉籽和棉铃中分离得到的链格孢(三个菌株)和细极链格孢(三个菌株)在各种实验室培养基上培养时具有产毒能力。通过溶剂分配、薄层色谱和仪器分析分离并鉴定出一种霉菌毒素为细交链孢菌酮酸。用卤虫和鸡胚生物测定法监测毒性。除链格孢938外,所有菌株在棉籽和酵母提取物 - 蔗糖肉汤上生长时均产生细交链孢菌酮酸。细极链格孢843在棉籽上产生的毒素最多(266毫克/千克)。