Salimova Dilara, Dalinova Anna, Dubovik Vsevolod, Senderskiy Igor, Stepanycheva Elena, Tomilova Oksana, Hu Qiongbo, Berestetskiy Alexander
Department of Phytotoxicology and Biotechnology, All-Russian Institute of Plant Protection, Podbelskogo Shosse, 3, Pushkin, 196608 Saint-Petersburg, Russia.
Institute of Systematics and Ecology of Animals SB RAS, Frunze Str. 11, 630091 Novosibirsk, Russia.
J Fungi (Basel). 2021 Sep 18;7(9):774. doi: 10.3390/jof7090774.
The study of fungal antibiotics in their competitive interactions with arthropods may lead to the development of novel biorational insecticides. Extracts of MFP253011 obtained using various methods showed a wide range of biological activities, including entomotoxic properties. Analysis of their composition and bioactivity allowed us to reveal several known mycotoxins and unidentified compounds that may be involved in the entomotoxic activity of the extracts. Among them, tenuazonic acid (TeA), which was the major component of the extracts, was found the most likely to have larvicidal activity against . In the intrahaemocoel injection bioassay, TeA was toxic to and of with an LT of 6 and 2 days, respectively, at the level of 50 µg/larva. Administered orally, TeA inhibited the growth of larvae and caused mortality of adults (LT 7 days) at a concentration of 250 µg/g of feed. TeA showed weak contact intestinal activity against the two phytophages, and , causing 15% and 27% mortality at a concentration of 1 mg/mL, respectively. TeA was cytotoxic to the Sf9 cell line (IC 25 µg/mL). Thus, model insects such as could be used for further toxicological characterization of TeA.
对真菌抗生素与节肢动物竞争相互作用的研究可能会促成新型生物合理杀虫剂的开发。使用各种方法获得的MFP253011提取物显示出广泛的生物活性，包括昆虫毒性特性。对其成分和生物活性的分析使我们能够揭示几种已知的霉菌毒素和可能参与提取物昆虫毒性活性的未鉴定化合物。其中，提取物的主要成分细交链孢菌酮酸（TeA）被发现最有可能对……具有杀幼虫活性。在血腔注射生物测定中，TeA对……有毒，在50μg/幼虫的剂量水平下，对……的LT50分别为6天和2天。口服时，TeA在250μg/g饲料的浓度下抑制……幼虫的生长并导致……成虫死亡（LT50为7天）。TeA对两种植食性昆虫……和……表现出较弱的接触肠道活性，在1mg/mL的浓度下分别导致15%和27%的死亡率。TeA对Sf9细胞系具有细胞毒性（IC50为25μg/mL）。因此，像……这样的模式昆虫可用于对TeA进行进一步的毒理学表征。
