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微小盘基网柄菌聚集敏感趋化剂的纯化与初步表征

Purification and preliminary characterization of an aggregation-sensitive chemoattractant of Dictyostelium minutum.

作者信息

Kakebeeke P I, Mato J M, Konijn T M

出版信息

J Bacteriol. 1978 Jan;133(1):403-5. doi: 10.1128/jb.133.1.403-405.1978.

Abstract

Aggregative amoebae of Dictyostelium minutum are not attracted by cyclic AMP; they are sensitive to various attracting sources from which yeast extract was chosen to purify the chemoattractant. A small acrasin-like species-specific molecule which contains glycine and C5H5N5 has been purified 30,000-fold. Several characteristics of this chemotactic molecule, which is inactivated by an enzyme that is not species specific, are described.

摘要

微小盘基网柄菌的聚集性变形虫不受环磷酸腺苷的吸引;它们对多种吸引源敏感,从中选择酵母提取物来纯化趋化剂。一种含有甘氨酸和C5H5N5的类似聚集素的小种特异性分子已被纯化了30000倍。描述了这种趋化分子的几个特征,它可被一种非物种特异性的酶灭活。

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