大鼠肝脏中丙酮酸（乙醛酸）氨基转移酶的亚细胞分布
Subcellular distribution of pyruvate (glyoxylate) aminotransferases in rat liver.
作者信息
Noguchi T, Minatogawa Y, Takada Y, Okuno E, Kido R
出版信息
Biochem J. 1978 Jan 15;170(1):173-5. doi: 10.1042/bj1700173.
Abstract
The distribution of pyruvate (glyoxylate) aminotransferases in the particulate fraction of rat liver homogenates was examined by centrifugation in a sucrose density graident. Aminotransferase activities towards serine, phenylalanine and histidine with pyruvate and those towards phenylalanine and histidine with glyoxylate were nearly identically distributed. Some 50-55% of the particulate activity was localized in the peroxisomes and the remainder in the mitochondria. Most of alanine-glyoxylate aminotransferase activity was localized in the mitochondria, with some activity in the peroxisomes. Glucagon injection resulted in increases of these enzyme activities in the mitochondria, but not in the peroxisomes.
摘要
通过在蔗糖密度梯度中离心，研究了大鼠肝脏匀浆颗粒部分中丙酮酸（乙醛酸）氨基转移酶的分布。以丙酮酸为底物时，对丝氨酸、苯丙氨酸和组氨酸的氨基转移酶活性，以及以乙醛酸为底物时，对苯丙氨酸和组氨酸的氨基转移酶活性，其分布几乎相同。约50 - 55%的颗粒活性定位于过氧化物酶体，其余定位于线粒体。大部分丙氨酸 - 乙醛酸氨基转移酶活性定位于线粒体，过氧化物酶体中也有一些活性。注射胰高血糖素导致线粒体中这些酶的活性增加，但过氧化物酶体中的活性未增加。
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本文引用的文献
1
Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles.
Biochem J. 1955 Mar;59(3):433-8. doi: 10.1042/bj0590433.
2
Liver-L-alanine-glyoxylate and L-serine-pyruvate aminotransferase activities: an apparent association with gluconeogenesis.
Biochem J. 1969 Dec;115(5):1071-3. doi: 10.1042/bj1151071.
3
Biosynthesis of the wall teichoic acid in Bacillus licheniformis.
Biochem J. 1972 Mar;127(1):27-37. doi: 10.1042/bj1270027.
4
The subcellular distribution of rat liver L-alanine-glyoxylate aminotransferase in relation to a pathway for glucose formation involving glyoxylate.
Biochem J. 1972 Mar;127(1):155-65. doi: 10.1042/bj1270155.
5
Colorimetric assay of catalase.
Anal Biochem. 1972 Jun;47(2):389-94. doi: 10.1016/0003-2697(72)90132-7.
6
Regulation of hepatic L-serine dehydratase and L-serine-pyruvate aminotransferase in the developing neonatal rat.
Biochem J. 1974 Dec;144(3):519-31. doi: 10.1042/bj1440519.
7
A comparison of the responses of mitochondrial and cytosol histidine-pyruvate aminotransferases to nutritional and hormonal treatments.
J Biol Chem. 1973 Feb 25;248(4):1459-65.
8
The adaptive behaviour of isoenzyme forms of rat liver alanine aminotransferases during development.
Biochem J. 1972 Jun;128(2):403-13. doi: 10.1042/bj1280403.
9
Mitochondrial-cytosolic interrelationships involved in gluconeogenesis from serine in rat liver.
FEBS Lett. 1975 Jul 15;55(1):202-5. doi: 10.1016/0014-5793(75)80992-6.
10
Purification, characterization and identification of rat liver histidine-pyruvate aminotransferase isoenzymes.
Biochem J. 1976 Apr 1;155(1):107-15. doi: 10.1042/bj1550107.
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