大鼠肝脏中丙酮酸（乙醛酸）氨基转移酶的亚细胞分布
Subcellular distribution of pyruvate (glyoxylate) aminotransferases in rat liver.
作者信息
Noguchi T, Minatogawa Y, Takada Y, Okuno E, Kido R
出版信息
Biochem J. 1978 Jan 15;170(1):173-5. doi: 10.1042/bj1700173.
Abstract
The distribution of pyruvate (glyoxylate) aminotransferases in the particulate fraction of rat liver homogenates was examined by centrifugation in a sucrose density graident. Aminotransferase activities towards serine, phenylalanine and histidine with pyruvate and those towards phenylalanine and histidine with glyoxylate were nearly identically distributed. Some 50-55% of the particulate activity was localized in the peroxisomes and the remainder in the mitochondria. Most of alanine-glyoxylate aminotransferase activity was localized in the mitochondria, with some activity in the peroxisomes. Glucagon injection resulted in increases of these enzyme activities in the mitochondria, but not in the peroxisomes.
摘要
通过在蔗糖密度梯度中离心，研究了大鼠肝脏匀浆颗粒部分中丙酮酸（乙醛酸）氨基转移酶的分布。以丙酮酸为底物时，对丝氨酸、苯丙氨酸和组氨酸的氨基转移酶活性，以及以乙醛酸为底物时，对苯丙氨酸和组氨酸的氨基转移酶活性，其分布几乎相同。约50 - 55%的颗粒活性定位于过氧化物酶体，其余定位于线粒体。大部分丙氨酸 - 乙醛酸氨基转移酶活性定位于线粒体，过氧化物酶体中也有一些活性。注射胰高血糖素导致线粒体中这些酶的活性增加，但过氧化物酶体中的活性未增加。
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