Nelson R A, Biro C E
Immunology. 1968 Apr;14(4):527-40.
A strain of complement (C′) deficient rabbits was studied. No C′ haemolytic activity was found in the sera of these rabbits. Both haemolytic and leucocidal activities were stored upon adding purified C′ isolated from either normal rabbits or normal guinea-pigs. The mixture of deficient serum with sensitized sheep cells resulted in the generation of active C′ sites, SAbC′ since lysis occurred after the addition of highly purified C′, C′, C′ and C′ to the washed cells. These rabbits possess a heat labile substance which is considerably heavier than C′. This C′ inactivator reacts specifically with the intermediate complex, EAbC′. The product of the reaction appears to be incapable of combining with the next component in the haemolytic sequence, C′. The inactivator is not depleted from the fluid phase during interaction with cells possessing active C′ sites. The elution characteristics from DEAE- and CM-cellulose and the sedimentation constants C′ and of the inactivator, as well as of certain other rabbit C′ components, were determined. C′ inactivator was also found in normal rabbit serum. A mechanism to explain the variability in results of homotransplant rejection in C′ deficient rabbits is offered.
对一组补体（C′）缺陷兔进行了研究。在这些兔的血清中未发现C′溶血活性。加入从正常兔或正常豚鼠分离的纯化C′后，溶血活性和杀白细胞活性均得以恢复。缺陷血清与致敏绵羊细胞的混合物导致活性C′位点SAbC′的产生，因为在向洗涤后的细胞中加入高度纯化的C′、C′、C′和C′后发生了细胞溶解。这些兔体内有一种热不稳定物质，其重量比C′大得多。这种C′灭活剂与中间复合物EAbC′发生特异性反应。反应产物似乎无法与溶血序列中的下一个成分C′结合。在与具有活性C′位点的细胞相互作用期间，灭活剂不会从液相中耗尽。测定了灭活剂以及某些其他兔C′成分从DEAE -纤维素和CM -纤维素上的洗脱特性以及沉降常数C′。在正常兔血清中也发现了C′灭活剂。提出了一种解释C′缺陷兔同种移植排斥结果变异性的机制。
