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牛基因组卫星DNA成分的限制性内切酶分析。

Restriction enzyme analysis of satellite DNA components from the bovine genome.

作者信息

Kopecka H, Macaya G, Cortadas J, Thiéry J P, Bernardi G

出版信息

Eur J Biochem. 1978 Mar;84(1):189-95. doi: 10.1111/j.1432-1033.1978.tb12156.x.

Abstract

A restriction enzyme analysis was performed on satellite DNA components, isolated, as described in the preceding paper, from the bovine genome by a combination of Cs2SO4/BAMD and Cs2SO4/Ag+ density gradient centrifugation. Such an analysis has led to the unambiguous identification of eight satellite DNA components and to new information on their repeat units; this indicates that identical repeat lengths are shared by them, a fact strongly suggesting a common origin.

摘要

如前文所述,通过硫酸铯/溴化乙锭密度梯度离心和硫酸铯/银离子密度梯度离心相结合的方法,从牛基因组中分离出卫星DNA组分,并对其进行了限制性内切酶分析。这样的分析明确鉴定出了8种卫星DNA组分,并获得了有关其重复单元的新信息;这表明它们具有相同的重复长度,这一事实强烈暗示了它们有共同的起源。

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