Crnic L S, Chase H P
J Nutr. 1978 Nov;108(11):1755-60. doi: 10.1093/jn/108.11.1755.
Although there are at least six commonly used methods for producing malnutrition in suckling rat pups, no attempt has been made to determine the quality of the nutrients available to the pups in each of these methods. In the present study, milk composition was determined for each of these methods and related to maternal nutritional status. These methods were: 1) large litters were fostered on the dams; 2) the dams were fed low protein diets; 3) the dams were fed a reduced amount of good quality diet; 4) and 5) the pups were removed from the dams and kept 4) with a non-lactating female or 5) in an incubator for half of the day; and 6) some of each dams' teats were destroyed. Maternal nutritional status was affected only in the cases where the dams' diet was deficient (methods 2 and 3), producing weight loss and lowered hematocrits in both cases and lowered serum protein when the low protein diet was fed. The low protein diet condition resulted in a lower nitrogen content and an elevated total fat content of the mild. It was concluded that previous consideration of all of the above methods as equivalent in terms of the quality of the milk produced is not justified.
虽然至少有六种常用方法可使哺乳大鼠幼崽出现营养不良，但尚未有人尝试确定这些方法中每种方法下幼崽可获得的营养物质质量。在本研究中，测定了这些方法中每种方法的乳汁成分，并将其与母体营养状况相关联。这些方法包括：1）将大量幼崽寄养在母鼠身上；2）给母鼠喂食低蛋白饮食；3）给母鼠喂食质量良好但数量减少的饮食；4）和5）将幼崽从母鼠身边带走，4）与非哺乳雌鼠一起饲养或5）在孵化器中饲养半天；6）破坏部分母鼠的乳头。仅在母鼠饮食缺乏的情况下（方法2和3），母体营养状况才受到影响，这两种情况均导致体重减轻和血细胞比容降低，喂食低蛋白饮食时血清蛋白也降低。低蛋白饮食状况导致乳汁中氮含量降低，总脂肪含量升高。得出的结论是，以往将上述所有方法在产生的乳汁质量方面视为等同是不合理的。
