Curran R C, Jones E L
J Pathol. 1978 May;125(1):39-51. doi: 10.1002/path.1711250107.
The distribution of immunoglobulin (Ig) in Hodgkin's tissue has been demonstrated in paraffin and cryostat sections by the unlabelled antibody peroxidase-antiperoxidase (PAP) method and the two-stage fluorescein isothiocyanate (FITC)-based technique. The morphology and histogenesis of the Reed-Sternberg (RS) and Hodgkin (HD) cells has been studied with the metalophil method of Marshall (1948) and this revealed a population of dendritic histiocytes which corresponded in number, sizes and distribution to the RS and HD cells in adjacent sections stained by haematoxylin and eosin. The largest RS cells, however, appeared to be non-dendritic. A notable feature of Hodgkin's tissue was the tendency for the dendritic cells to form "nodules", in combination with a population of small lymphocytes. The PAP technique reveals Ig in the form of mu and delta heavy chains, as well as kappa and lambda light chains, in close association with and probably on the surface of a high proportion of these lymphocytes, suggesting that they are immature B cells. Similar reactions were given by the mantle of lymphocytes of surviving normal lymphoid follicles, and metalophil dendritic histiocytes were also demonstrated within the mantle and subjacent part of the germinal centre. Numerous immunocytes containing Ig were present in all lesions; in the majority of cases, more cells contained gamma than alpha or mu heavy chains, although in these cases alpha-positive cells outnumbered those containing gamma or mu heavy chains. In two-thirds of the cases, one-third of the RS cells contained cytoplasmic immunoglobulin. This was exclusively gamma heavy, although both light chains were present. In about half the cases a minority of the HD cells contained gamma chain. The results suggest that HD and RS cells are dendritic histiocytes of the type normally found in the lymphoid follicles and that their tendency to form nodules in assoication with B lymphocytes is a manifestatin of this origin. It is suggested that the presence of Ig most probably results from absorption of antigen-antibody complexes on the cell surface.
采用未标记抗体过氧化物酶 - 抗过氧化物酶（PAP）法以及基于异硫氰酸荧光素（FITC）的两步法，已在石蜡切片和低温切片中证实了霍奇金组织中免疫球蛋白（Ig）的分布。采用马歇尔（1948年）的嗜金属法研究了里德 - 施特恩伯格（RS）细胞和霍奇金（HD）细胞的形态及组织发生，结果显示一群树突状组织细胞，其数量、大小和分布与苏木精和伊红染色的相邻切片中的RS细胞和HD细胞相对应。然而，最大的RS细胞似乎不是树突状的。霍奇金组织的一个显著特征是树突状细胞倾向于与一群小淋巴细胞结合形成“结节”。PAP技术显示，在这些淋巴细胞的很大一部分表面或与之紧密相关处，存在以μ和δ重链以及κ和λ轻链形式存在的Ig，这表明它们是未成熟的B细胞。存活的正常淋巴滤泡的淋巴细胞套也有类似反应，并且在生发中心的套层和下层也证实有嗜金属树突状组织细胞。所有病变中均存在大量含Ig的免疫细胞；在大多数病例中，含γ重链的细胞比含α或μ重链的细胞多，尽管在这些病例中α阳性细胞的数量超过含γ或μ重链的细胞。在三分之二的病例中，三分之一的RS细胞含有细胞质免疫球蛋白。其仅为γ重链，不过两种轻链均存在。在大约一半的病例中，少数HD细胞含有γ链。结果表明，HD细胞和RS细胞是通常在淋巴滤泡中发现的那种树突状组织细胞，并且它们与B淋巴细胞结合形成结节的倾向是这种起源的一种表现。有人认为，Ig的存在很可能是由于抗原 - 抗体复合物在细胞表面的吸附所致。
