Gordis E
J Clin Invest. 1969 Jan;48(1):203-9. doi: 10.1172/JCI105969.
5 min after intravenous injection into rats of (14)C- or (36)Cl-carbon tetrachloride, liver lipids were found labeled. Most of the radioactivity was found in the phospholipid fraction. The metabolites were shown to comprise a heterogeneous group of branched long-chain chlorinated fatty acids, probably containing the trichloromethyl side chain. Surviving liver slices also formed these metabolites. In a simple chemical system which generates trichloromethyl free radicals, carbon tetrachloride added to methyl oleate to form esters which behaved like the metabolites during counter-current distribution and urea adduction. The evidence strongly suggests the formation of these metabolites by free radical attack on unsaturated lipids. The relation of these observations to current theories of carbon tetrachloride intoxication is discussed.
给大鼠静脉注射（14）C或（36）Cl标记的四氯化碳5分钟后，发现肝脏脂质被标记。大部分放射性存在于磷脂部分。这些代谢产物被证明是一组异质的支链长链氯化脂肪酸，可能含有三氯甲基侧链。存活的肝切片也能形成这些代谢产物。在一个能产生三氯甲基自由基的简单化学体系中，将四氯化碳加入油酸甲酯中形成酯，这些酯在逆流分配和尿素加成过程中的行为与代谢产物相似。有力的证据表明，这些代谢产物是自由基对不饱和脂质攻击形成的。本文讨论了这些观察结果与当前四氯化碳中毒理论的关系。
