Perry D
J Bacteriol. 1969 Feb;97(2):518-21. doi: 10.1128/jb.97.2.518-521.1969.
Three streptomycin-resistant mutants of group H streptococcus, strain Challis, were examined for ability to accumulate (14)C-streptomycin. Although the mutants exhibited different levels of transformation, only the streptomycin-sensitive parent Challis strain accumulated significant amounts of (14)C-streptomycin. It appears that impermeability to streptomycin does not necessarily result in reduction or loss of transformability. The amount of label accumulated by strain Challis was correlated with a loss of viability. In addition, accumulation of label was influenced by the concentration of (14)C-streptomycin, the time of exposure, and the type of medium employed.
对H群链球菌Challis菌株的三个链霉素抗性突变体进行了积累¹⁴C-链霉素能力的检测。尽管这些突变体表现出不同水平的转化能力，但只有链霉素敏感的亲本Challis菌株积累了大量的¹⁴C-链霉素。看来对链霉素的不透性不一定导致转化能力的降低或丧失。Challis菌株积累的标记量与活力丧失相关。此外，标记的积累受¹⁴C-链霉素浓度、暴露时间和所用培养基类型的影响。
