Berns K I, Silverman C, Weissbach A
J Virol. 1969 Jul;4(1):15-23. doi: 10.1128/JVI.4.1.15-23.1969.
HeLa cell deoxyribonucleic acid (DNA) polymerase was purified about 100-fold by sequential column chromatography on phosphocellulose, hydroxylapatite, and Bio Rex 70. A new form of DNA polymerase found in vaccinia virus-infected cells was separated from HeLa DNA polymerase by chromatography on diethylamino-ethyl cellulose. The new form was also purified approximately 100-fold in the same manner as the HeLa DNA polymerase. In addition to chromatographic differences, the two enzymes differed with regard to primer response, relative activity at high pH, inactivation by heat and p-chloromercuribenzoate, and inhibition by vaccinia antiserum.
通过先后在磷酸纤维素柱、羟基磷灰石柱和Bio Rex 70柱上进行层析，将海拉细胞脱氧核糖核酸（DNA）聚合酶纯化了约100倍。通过在二乙氨基乙基纤维素柱上层析，从海拉DNA聚合酶中分离出痘苗病毒感染细胞中发现的一种新形式的DNA聚合酶。这种新形式的酶也以与海拉DNA聚合酶相同的方式纯化了约100倍。除了层析差异外，这两种酶在引物反应、高pH值下的相对活性、热和对氯汞苯甲酸的失活作用以及痘苗抗血清的抑制作用方面也有所不同。
