Arndt D, Schwalbe P
Acta Biol Med Ger. 1978;37(7):1113-23.
The effects of supernatants of primary and secondary malignant human lymphoma cell cultures were analyzed as parameters of spontaneous secretion of factors by these cells using the leukocyte migration test (LMT). Spontaneous cultivation for up to five weeks was successful in four cases. The postulated production of mediators, i.e. the inhibitory and stimulating effects on leukocyte migration were characterized by testing the influence of (a) concentration, (b) temperature and (c) absorption with normal blood leukocytes on the effect. Reproducible stimulatory and inhibitory effects on the migration of normal leukocytes were dependent on concentration and temperature and were apparently mediated by one or more factors. The supernatants of a lymphoblastic lymphosarcoma of the T-cell type and of a lymphoblastic lymphosarcoma clearly revealed congruous and reproducible inhibitory effects. A further case of lymphoblastic lymphosarcoma that could not exactly be defined with immunological methods either and a case of centroblastic/centrocytic lymphosarcoma exhibited stimulating effects which could be reduced in a time-dependent manner through preincubation with blood leukocytes. The results of these studies support the assumption that malignant lymphoma cells are capable not only of secreting immunoglobulin, but also of other biologically effective secretion. The effects of such secretion are differentiated into stimulating and inhibitory ones. They might be important for the spreading of a tumor or for resistance of the organism to the disease.
使用白细胞迁移试验（LMT），分析原发性和继发性人类恶性淋巴瘤细胞培养上清液的作用，以此作为这些细胞自发分泌因子的参数。在4例病例中，成功进行了长达5周的自发培养。通过测试（a）浓度、（b）温度和（c）与正常血液白细胞的吸附对效应的影响，来表征假定的介质产生，即对白细胞迁移的抑制和刺激作用。对正常白细胞迁移的可重复刺激和抑制作用取决于浓度和温度，并且显然由一种或多种因子介导。T细胞型淋巴母细胞性淋巴肉瘤和淋巴母细胞性淋巴肉瘤的上清液清楚地显示出一致且可重复的抑制作用。另一例无法用免疫学方法确切定义的淋巴母细胞性淋巴肉瘤病例以及一例中心母细胞/中心细胞性淋巴肉瘤病例表现出刺激作用，通过与血液白细胞预孵育，这种刺激作用可随时间降低。这些研究结果支持这样的假设，即恶性淋巴瘤细胞不仅能够分泌免疫球蛋白，还能够进行其他具有生物学效应的分泌。这种分泌的作用可分为刺激作用和抑制作用。它们可能对肿瘤的扩散或机体对疾病的抵抗力很重要。
