Ugai K, Ziff M, Jasin H E
Arthritis Rheum. 1979 Apr;22(4):353-64. doi: 10.1002/art.1780220407.
The present experiments were designed to investigate in vitro interactions between polymorphonuclear leukocytes (PMN) and rabbit joint collagenous tissues containing trapped immune complexes. Articular cartilages and menisci from antigen-injected and control joints were incubated with normal PMN isolated from rabbit peritoneal exudates or blood. After incubation of cartilage and menisci from antigen-injected joints with PMN, large numbers of PMN became attached to the articular surface. In areas of superficial erosion, the PMN invaded the tissue several cell diameters below the articular surface. Through immunoelectron microscopy, degranulated PMN were observed in scattered areas to phagocytose amorphous material containing rabbit Ig. Following addition of PMN to control tissues, only a few PMN became attached to the articular surface. When tissues from monosodium urate-injected joints were incubated with PMN, these cells were found attached to the surface in moderate numbers, but invasion into the tissues was not seen. These studies indicate that immune complexes trapped in joint collagenous tissues may lead to enhanced release of lysosomal hydrolases.
本实验旨在研究多形核白细胞（PMN）与含有捕获免疫复合物的兔关节胶原组织之间的体外相互作用。将来自抗原注射关节和对照关节的关节软骨和半月板与从兔腹膜渗出液或血液中分离出的正常PMN一起孵育。用PMN孵育来自抗原注射关节的软骨和半月板后，大量PMN附着于关节表面。在浅表侵蚀区域，PMN侵入关节表面以下几个细胞直径的组织。通过免疫电子显微镜观察，在散在区域观察到脱颗粒的PMN吞噬含有兔Ig的无定形物质。将PMN添加到对照组织后，只有少数PMN附着于关节表面。当将来自注射尿酸钠关节的组织与PMN一起孵育时，发现这些细胞以中等数量附着于表面，但未观察到侵入组织的情况。这些研究表明，捕获在关节胶原组织中的免疫复合物可能导致溶酶体水解酶的释放增加。
