Pulmonary responses to atmospheric pollutants. II. Effect of petrol vapour inhalation on secretion of pulmonary surfactant.

Inhalation of air contaminated with petrol vapour has been shown to produce reduced surfactant levels in the lungs of rats. Pulmonary surfactant was obtained by endobronchial lavage followed by salt extraction and freeze drying to obtain the dry, hydrophobic product. During 45 days of continuous exposure, the lowest yield of surfactant was obtained after 15 days of treatment. During the following 30 days of treatment, the surfactant yield reached a relatively constant level, approximately half the mean value for control animals. Chromatographic analysis indicated no qualitative alteration in the phospholipid components of surfactant with increasing times of exposure to the irritant. It has been possible to correlate biochemical evidence of toxic lung injury with signs of cellular damage obtained from ultrastructural studies.