Le Mesurier S M, Stewart B W, O'Connell P J, Lykke A W
Pathology. 1979 Jan;11(1):81-7. doi: 10.3109/00313027909063542.
Inhalation of air contaminated with petrol vapour has been shown to produce reduced surfactant levels in the lungs of rats. Pulmonary surfactant was obtained by endobronchial lavage followed by salt extraction and freeze drying to obtain the dry, hydrophobic product. During 45 days of continuous exposure, the lowest yield of surfactant was obtained after 15 days of treatment. During the following 30 days of treatment, the surfactant yield reached a relatively constant level, approximately half the mean value for control animals. Chromatographic analysis indicated no qualitative alteration in the phospholipid components of surfactant with increasing times of exposure to the irritant. It has been possible to correlate biochemical evidence of toxic lung injury with signs of cellular damage obtained from ultrastructural studies.
吸入被汽油蒸汽污染的空气已被证明会使大鼠肺部表面活性剂水平降低。通过支气管内灌洗，随后进行盐提取和冷冻干燥以获得干燥的疏水性产物，从而获取肺表面活性剂。在连续暴露的45天里，处理15天后表面活性剂的产量最低。在随后的30天处理过程中，表面活性剂产量达到相对稳定的水平，约为对照动物平均值的一半。色谱分析表明，随着暴露于刺激物时间的增加，表面活性剂的磷脂成分没有质的改变。已经能够将有毒肺损伤的生化证据与超微结构研究获得的细胞损伤迹象联系起来。
