van der Waaij D, Tieleman-Speltie T M, de Roeck-Houben A M
Antonie Van Leeuwenhoek. 1978;44(3-4):395-405. doi: 10.1007/BF00394316.
Lethally irradiated conventional mice were killed at daily intervals after irradiation. A high correlation was found between the occurrence of bacterial overgrowth in the digestive tract and invasion into the regional lymph nodes the spleen and the blood. By oral contamination of mice with quite high doses of either an exogenous or an endogenous biotype of an Enterobacteriaceae species at day 4 after irradiation, it was attempted to induced such condition of intestinal overgrowth. In all animals such an abnormal colonization of the contaminant was indeed achieved in this way and the mice died from an infection caused by the biotype used for the contamination. The interval between contamination and death was different when different biotypes were used for contamination and occurred sooner after irradiation when an endogenous biotype was used for (re)contamination. It is concluded that oral contamination with exogenous as well as with endogenous potentially pathogenic bacteria during a period of severely decreased resistance to infections must be avoided. The colonization resistance of the digestive tract of these animals should be maintained as high as possible.
对致死剂量照射后的常规小鼠每天定时处死。结果发现，消化道细菌过度生长的发生与细菌侵入局部淋巴结、脾脏及血液之间存在高度相关性。在照射后第4天，通过给小鼠经口接种相当高剂量的肠杆菌科细菌的外源性或内源性生物型，试图诱发肠道过度生长的这种情况。通过这种方式，确实在所有动物中实现了污染物的这种异常定植，并且小鼠死于由用于污染的生物型引起的感染。当使用不同生物型进行污染时，污染与死亡之间的间隔不同，并且当使用内源性生物型进行（再）污染时，在照射后死亡更快发生。结论是，在对感染的抵抗力严重下降的时期，必须避免经口感染外源性以及内源性潜在致病菌。应尽可能保持这些动物消化道的定植抗力。
