Cave D R, Mitchell D N, Brooke B N
Lancet. 1976 Jun 19;1(7973):1311-5. doi: 10.1016/s0140-6736(76)92649-0.
Five New Zealand White rabbits were injected intracolonically with homogenates (100 mum) of ulcerative-colitis tissue. Histological changes closely similar to those seen in the human donors were present in the mucosa and submucosa of the large intestine of four of these rabbits 3-12 months later. Similar changes were seen in the large intestine of three of four rabbits 6-13 months after intravenous or intracolonic inoculation of homogenates of rabbit mesenteric lymph-nodes after passage of human ulcerative colitis tissue (100 mum or 0.2 mum). Three of thirty A2G strain mice injected with similar tissue homogenates (100 mum or 0.2 mum) from patients with ulcerative colitis into the footpad or intraperitoneally, had granulomatous changes in footpad, bowel, liver and/or spleen 3-22 months later. Such changes did not develop in rabbits or mice inoculated with tissue from normal controls. The results of these experiments suggest that a transmissible factor is involved in the aetiology of ulcerative colitis.
五只新西兰白兔经结肠内注射溃疡性结肠炎组织匀浆（100微米）。3至12个月后，其中四只兔子大肠的黏膜和黏膜下层出现了与人类供体中所见极为相似的组织学变化。在人类溃疡性结肠炎组织（100微米或0.2微米）传代后，对四只兔子静脉内或结肠内接种兔肠系膜淋巴结匀浆，6至13个月后，其中三只兔子的大肠出现了类似变化。将溃疡性结肠炎患者的类似组织匀浆（100微米或0.2微米）经足垫或腹腔内注射到30只A2G品系小鼠中，3至22个月后，其中三只小鼠的足垫、肠道、肝脏和/或脾脏出现了肉芽肿性变化。接种正常对照组织的兔子或小鼠未出现此类变化。这些实验结果表明，溃疡性结肠炎的病因中涉及一种可传播因子。
