噬菌体T-4中的紫外线诱变。I. 细胞外噬菌体颗粒的辐照。
Ultraviolet mutagenesis in bacteriophage T-4. I. Irradiation of extracellular phage particles.
作者信息
Drake J W
出版信息
J Bacteriol. 1966 May;91(5):1775-80. doi: 10.1128/jb.91.5.1775-1780.1966.
Abstract
Drake, John W. (University of Illinois, Urbana). Ultraviolet mutagenesis in bacteriophage T4. I. Irradiation of extracellular phage particles. J. Bacteriol. 91:1775-1780. 1966.-Ultraviolet (UV) irradiation of extracellular T4 phage particles induces about 2 x 10(-4)r mutations per lethal hit. The mutants largely escape detection unless the irradiated phages are plated with very soft overlay agar. Multiplicity reactivation is not a prerequisite for mutagenesis. A much higher frequency of base pair substitution-type mutants is induced than is found in the spontaneous background, but sign mutants are also induced. Nearly half of the mutants map into previously identified UV hot spots. The rII mutants induced extracellularly are very similar to those induced intracellularly. The mutants also appear to result from direct radiation effects upon the bacteriophage deoxyribonucleic acid.
摘要
德雷克，约翰·W.（伊利诺伊大学厄巴纳分校）。噬菌体T4中的紫外线诱变。I. 细胞外噬菌体颗粒的照射。《细菌学杂志》91:1775 - 1780。1966年。——对细胞外T4噬菌体颗粒进行紫外线（UV）照射，每致死一击诱导约2×10⁻⁴个r突变。除非将照射过的噬菌体接种在非常软的上层琼脂上，否则这些突变体很难被检测到。多重复活不是诱变的先决条件。与自发背景相比，诱导产生的碱基对替换型突变体的频率要高得多，但也会诱导产生sign突变体。近一半的突变体定位到先前确定的UV热点区域。在细胞外诱导产生的rII突变体与在细胞内诱导产生的非常相似。这些突变体似乎也是噬菌体脱氧核糖核酸受到直接辐射效应的结果。
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