大鼠小肠中的流动电位。

Streaming potentials in the rat small intestine.

作者信息

Smyth D H, Wright E M

出版信息

J Physiol. 1966 Feb;182(3):591-602. doi: 10.1113/jphysiol.1966.sp007839.

DOI:10.1113/jphysiol.1966.sp007839

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1357489/

Abstract

The effect of adverse osmotic pressure gradients on fluid transfer and electrical potential across the wall of sacs of rat everted small intestine was investigated.2. Addition of mannitol to the mucosal fluid produced a potential change of 0.062 mV/m-osM and a decrease in fluid transfer of 0.015 ml./m-osM/hr. This is consistent with the production of streaming potentials due to fluid movement through negatively charged pores in the intestine.3. The solute-linked fluid movement does not pass through these negatively charged pores which are responsible for the streaming potentials.4. From the magnitude and polarity of the streaming potential a value of -50 mV has been calculated for the zeta potential at the phase boundary in the pores.5. Streaming potentials have been used to measure the equivalent pore radius, and a value of 4A has been obtained.6. It is concluded that electro-osmosis is not responsible for fluid transfer by the intestine, and the potential difference associated with hexose transfer is not electrokinetic in origin.

摘要

研究了不利的渗透压梯度对大鼠外翻小肠囊壁上液体转移和电位的影响。
向黏膜液中添加甘露醇会产生0.062 mV/m-osM的电位变化以及0.015 ml./m-osM/hr的液体转移减少。这与由于液体通过肠道中带负电荷的孔隙移动而产生的流动电位一致。
溶质相关的液体移动不通过这些负责流动电位的带负电荷的孔隙。
根据流动电位的大小和极性，计算出孔隙中相界面处的ζ电位值为-50 mV。
流动电位已被用于测量等效孔隙半径，并获得了4A的值。
得出的结论是，电渗作用不是肠道液体转移的原因，与己糖转移相关的电位差在起源上不是电动的。