Keller K H, Friedlander S K
J Gen Physiol. 1966 Mar;49(4):663-79. doi: 10.1085/jgp.49.4.663.
The steady-state transport of oxygen through hemoglobin solutions was studied to identify the mechanism of the diffusion augmentation observed at low oxygen tensions. A novel technique employing a platinum-silver oxygen electrode was developed to measure the effective diffusion coefficient of oxygen in steady-state transport. The measurements were made over a wider range of hemoglobin and oxygen concentrations than previously reported. Values of the Brownian motion diffusion coefficient of oxygen in hemoglobin solution were obtained as well as measurements of facilitated transport at low oxygen tensions. Transport rates up to ten times greater than ordinary diffusion rates were found. Predictions of oxygen flux were made assuming that the oxyhemoglobin transport coefficient was equal to the Brownian motion diffusivity which was measured in a separate set of experiments. The close correlation between prediction and experiment indicates that the diffusion of oxyhemoglobin is the mechanism by which steady-state oxygen transport is facilitated.
研究了氧气在血红蛋白溶液中的稳态传输，以确定在低氧张力下观察到的扩散增强机制。开发了一种采用铂银氧电极的新技术，用于测量稳态传输中氧气的有效扩散系数。测量范围比以前报道的更宽，涵盖了更广泛的血红蛋白和氧气浓度。获得了氧气在血红蛋白溶液中的布朗运动扩散系数值，以及在低氧张力下促进传输的测量结果。发现传输速率比普通扩散速率高十倍。假设氧合血红蛋白传输系数等于在另一组实验中测量的布朗运动扩散率，对氧气通量进行了预测。预测与实验之间的密切相关性表明，氧合血红蛋白的扩散是促进稳态氧气传输的机制。
