从公羊睾丸中纯化β-乙酰氨基葡萄糖苷酶和β-半乳糖苷酶。
Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.
作者信息
Caygill J C, Roston C P, Jevons F R
出版信息
Biochem J. 1966 Feb;98(2):405-9. doi: 10.1042/bj0980405.
Abstract
- The presence of beta-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of beta-acetylglucosaminase (EC 3.2.1.30) and of beta-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the beta-acetylglucosaminase 35 times and for the beta-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.
摘要
- 据报道，在公羊睾丸的乙酸提取物中存在β-半乳糖苷酶（EC 3.2.1.23）。对粗酶制剂的一些特性进行了研究。2. 描述了通过硫酸铵沉淀和在CM-纤维素柱上进行色谱法从公羊睾丸提取物中纯化β-N-乙酰氨基葡萄糖苷酶（EC 3.2.1.30）和β-半乳糖苷酶的方法。3. 分离得到的酶最终纯化倍数，β-N-乙酰氨基葡萄糖苷酶为35倍，β-半乳糖苷酶为99倍。4. 研究了使用DEAE-纤维素和葡聚糖凝胶G-200纯化这些酶的可能性。
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