Koga S, Echigo A, Nunomura K
Biophys J. 1966 Sep;6(5):665-74. doi: 10.1016/S0006-3495(66)86685-7.
The equilibrium vapor pressure, the heat of vaporization, the dielectric increment, and the NMR spectra of partially dried cells were studied in Saccharomyces cerevisiae with water contents varying in the range from 25 to 0.8%. The comparative study of those physical properties suggests that physical states of the microbe can be classified into four regions in accordance with the states of the cell water: the solution region, the gel region, the mobile adsorption region, and the localized water region. Much difference in the physiological properties is found between the cells in the solution region and those in the gel region, whereas the pattern changes in physical properties take place when the cells in the gel region are dried to a further extent into the mobile or the localized region. The various modes in the molecular motion of the cell water reflected in those physical properties of the cell seem to give some insight into the biological functions of the molecule in the native as well as the dried states of the cell.
研究了酿酒酵母中水分含量在25%至0.8%范围内的部分干燥细胞的平衡蒸气压、汽化热、介电增量和核磁共振谱。对这些物理性质的比较研究表明，根据细胞水的状态，微生物的物理状态可分为四个区域：溶液区域、凝胶区域、移动吸附区域和局部水区域。溶液区域的细胞与凝胶区域的细胞在生理特性上存在很大差异，而当凝胶区域的细胞进一步干燥到移动或局部区域时，物理性质会发生模式变化。细胞的这些物理性质所反映的细胞水的分子运动的各种模式，似乎为了解细胞天然状态和干燥状态下分子的生物学功能提供了一些线索。
