以高产量和高比活性合成脊髓灰质炎病毒RNA的大量、可能完整的DNA拷贝。
Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.
作者信息
Kacian D L, Myers J C
出版信息
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2191-5. doi: 10.1073/pnas.73.7.2191.
Abstract
The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of two size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.
摘要
本文描述了用禽成髓细胞瘤病毒DNA聚合酶合成脊髓灰质炎病毒RNA的大片段、可能是完整的互补DNA（cDNA）拷贝的过程。该cDNA由两个大小类别组成，其中较大的约为7500个核苷酸。在过量的脱氧核苷三磷酸、核糖核苷三磷酸或焦磷酸钠存在的情况下，只能获得较大的片段。大片段cDNA的产量为输入RNA的50-75%。
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Proc Natl Acad Sci U S A. 1976 Jul;73(7):2191-5. doi: 10.1073/pnas.73.7.2191.
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