脊髓灰质炎病毒RNA大量DNA拷贝合成过程中产生的较小DNA的抗补体性质。
Anticomplementary nature of smaller DNA produced during synthesis of extensive DNA copies of poliovirus RNA.
作者信息
Kacian D L, Myers J C
出版信息
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3408-12. doi: 10.1073/pnas.73.10.3408.
Abstract
The reverse transcriptase (RNA-directed DNA nucleotidyltransferase) from avian myeloblastosis virus is able to make an extensive, possibly complete, complementary DNA copy of intact poliovirus RNA. In the presence of high concentrations of deoxyribonucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, this DNA is the only species produced. Without these additives, however, a second size class of DNA is also synthesized. This material has a sedimentation coefficient between roughly 4 and 10 S and is produced later in the reaction, largely after synthesis of the larger complementary DNA has ceased. The smaller DNA consists primarily of material anticomplementary to the RNA template and contains a faithful and uniform representation of the viral sequences. It most likely arises by transcription of the larger DNA species.
摘要
禽成髓细胞瘤病毒的逆转录酶（RNA 指导的 DNA 核苷酸转移酶）能够对完整的脊髓灰质炎病毒 RNA 进行广泛的、可能是完整的互补 DNA 复制。在高浓度脱氧核糖核苷三磷酸、核糖核苷三磷酸或焦磷酸钠存在的情况下，这种 DNA 是唯一产生的产物。然而，没有这些添加剂时，还会合成第二种大小类别的 DNA。这种物质的沉降系数大致在 4 到 10 S 之间，在反应后期产生，主要是在较大的互补 DNA 合成停止之后。较小的 DNA 主要由与 RNA 模板反互补的物质组成，并且包含病毒序列的忠实且一致的呈现。它很可能是由较大的 DNA 种类转录产生的。
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Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.以高产量和高比活性合成脊髓灰质炎病毒RNA的大量、可能完整的DNA拷贝。
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