癌症患者在MEM测试中的反应：不仅仅是碱性蛋白质电荷的函数。
Responses of cancer patients in the MEM test: not just a function of charge on basic proteins.
作者信息
Shaw A, Ettin G, McPherson T A
出版信息
Br J Cancer. 1976 Jul;34(1):7-13. doi: 10.1038/bjc.1976.114.
It has been reported that lymphocytes from cancer patients give positive responses to PPD, myelin basic protein, tumour basic protein, and certain histone fractions in the MEM test. The underlying mechanisms of the MEM test are poorly understood, but it is widely assumed that it detects immunological sensitization to specific antigenic determinants. The cross-reactivity experienced is interpreted as indicating shared antigenicity. Since all the stimulatory proteins are strongly basic we investigated an alternative explanation that responsiveness is a function of electrical charge by comparing the known stimulatory proteins in the MEM test with two others of similar basicity: lysozyme and cytochrome-C. We obtained highly significant stimulation with PPD, tryptophane peptide of myelin, and tumour basic protein using Mantoux + cancer patients, but found no response to other basic proteins. We failed to confirm the reported activity of histone F2a. Our results indicate that basicity alone is insufficient to elicit response, and strengthens the concept that the MEM test is measuring sensitization to the determinants shared by myelin and tumour basic protein.
据报道，癌症患者的淋巴细胞在微量淋巴细胞毒试验（MEM试验）中对结核菌素纯蛋白衍生物（PPD）、髓鞘碱性蛋白、肿瘤碱性蛋白及某些组蛋白组分呈阳性反应。MEM试验的潜在机制尚不清楚，但人们普遍认为它能检测出对特定抗原决定簇的免疫致敏。所经历的交叉反应被解释为表明存在共同抗原性。由于所有刺激性蛋白都具有强碱性，我们通过将MEM试验中已知的刺激性蛋白与另外两种碱性相似的蛋白：溶菌酶和细胞色素C进行比较，研究了另一种解释，即反应性是电荷的函数。我们使用曼托试验阳性的癌症患者，发现PPD、髓鞘色氨酸肽和肿瘤碱性蛋白能产生高度显著的刺激，但对其他碱性蛋白没有反应。我们未能证实所报道的组蛋白F2a的活性。我们的结果表明，仅碱性不足以引发反应，并强化了MEM试验正在检测对髓鞘碱性蛋白和肿瘤碱性蛋白共有的决定簇的致敏这一概念。
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