Warnatz H, Gutmann W, Rösch W, Hommel G
Z Immunitatsforsch Immunobiol. 1977 Dec;153(5):435-49.
The cytotoxicity of lymphocytes from patients with chronic active hepatitis, chronic persistent hepatitis, acute hepatitis B and rheumatoid arthritis as well as from normal controls was studied in a microcytotoxicity assay according to COHEN et al. using 125I-iododeoxyuridine labeled embryonal liver cells and Chang cells as target cells. Unfractionated lymphocytes of the peripheral blood from patients with chronic active hepatitis and rheumatoid arthritis showed a high frequency of cytotoxic activity. The lymphocytotoxicity in chronic active hepatitis was significantly increased in comparison to normal controls at the EC/TC of 10:1 and 100:1. Specificity of the cytotoxic reaction to target cells could not be demonstrated. Addition of autologous serum to the cytotoxic assay blocked the lymphocytotoxicity in patients with chronic active hepatitis. A weak potentiating effect on lymphocytotoxicity was observed in patients with hepatitis B after addition of autologous serum. It is discussed that this reaction is due to the presence of HB-antigen in the serum since addition of HB-antigen from other sources increased also the lymphocytotoxicity in hepatitis B patients. This effect was observed neither in HB-antigen positive nor in HB-antigen negative patients with chronic active hepatitis or chronic persistent hepatisis.
根据科恩等人的方法，采用微量细胞毒性试验，以125I-碘脱氧尿苷标记的胚胎肝细胞和张氏细胞作为靶细胞，研究慢性活动性肝炎、慢性持续性肝炎、急性乙型肝炎和类风湿性关节炎患者以及正常对照者淋巴细胞的细胞毒性。慢性活动性肝炎和类风湿性关节炎患者外周血未分离的淋巴细胞显示出高频率的细胞毒性活性。在效靶比为10:1和100:1时，慢性活动性肝炎患者的淋巴细胞毒性与正常对照相比显著增加。未证实细胞毒性反应对靶细胞具有特异性。在细胞毒性试验中加入自体血清可阻断慢性活动性肝炎患者的淋巴细胞毒性。在乙型肝炎患者中加入自体血清后，观察到对淋巴细胞毒性有微弱的增强作用。有人认为这种反应是由于血清中存在乙肝抗原，因为加入其他来源的乙肝抗原也会增加乙型肝炎患者的淋巴细胞毒性。在慢性活动性肝炎或慢性持续性肝炎的乙肝抗原阳性和阴性患者中均未观察到这种效应。
