一种通过聚乙二醇使悬浮细胞融合的方法。
A procedure for the fusion of cells in suspension by means of polyethylene glycol.
作者信息
Hales A
出版信息
Somatic Cell Genet. 1977 Mar;3(2):227-30. doi: 10.1007/BF01551817.
PMID:605382
Abstract
The nuclei of chick and human fibroblasts are readily distinguishable in Leishman stained heterokaryons. The use of these two types of cell has allowed development of a method for fusion in suspension based on the use of polyethylene glycol (PEG) of MW 1000 in Dulbecco's medium containing 15% dimethylsulfoxide (DMSO).
摘要
在利什曼染色的异核体中，鸡和人类成纤维细胞的细胞核很容易区分。使用这两种类型的细胞，基于在含有15%二甲基亚砜（DMSO）的杜尔贝科培养基中使用分子量为1000的聚乙二醇（PEG），开发出了一种悬浮融合方法。
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